Prof. Dr. med. Thomas Tüting (head of research group) and Dr. Tetje C. van der Sluis, from the Department of Experimental Dermatology at University Clinic of Dermatology and Allergy Magdeburg are looking for a PhD Student (start as soon as possible) to a research project looking into the role of CD4+ T cells in tumor immunity. The aim of the project is to dissect the effector mechanisms of antitumoral CD4+ T cells through spatio-temporal analyses. This research will be conducted in the context of the research and teaching program of the Collaborative Research Centre 854 “Molecular organization of cellular communication within the immune system”.
Over the last two decades, it has become very clear that the body’s own immune system can recognize and kill tumor cells. Immunotherapy has become a very active area of cancer research and up today, countless different type of immunotherapies exists. In addition to the cytotoxic CD8+ T cells, also CD4+ T cells play an important role in anti-tumor immune responses. However, little is known about the interactions of the CD4+ T cells with other type of immune cells and tumor cells within the tumor microenvironment and how this affects their function.
Utilizing ten-color flow cytometry and different types of optical microscopy (including intravital microscopy), the candidate will follow the differentiation path of CD4+ T cells and neutrophils within experimental mouse tumors. The candidate will examine the phenotypic plasticity of the CD4+ T cells and the possibilities to reinvigorate them through combination therapies and booster vaccinations. Using multiple neutrophil as well as regulatory T cell depleting methods the role of neutrophils and regulatory T cells on CD4+ T cell differentiation and function will be studied.
Finally, the candidate will use CRISPR/Cas9 knock-in strategies to tag endogenous genes in the melanoma genome. This allows the monitoring of antigen expression levels as well as the potential development of antigen-loss variants.
- A challenging and inspiring project in the PhD training program of the CRC854 in a research group that has an excellent publication record.
- Doctoral position (65%; TV-L E13) in a 4-year research project.
- Research fields: cancer immunology, virology, melanoma and advanced mouse models.
- Laboratory techniques: cell culture, mouse studies, multicolor flow cytometry, intravital microscopy, fluorescent microscopy, confocal microscopy, qPCR, Western blot, expansion and purification of adenoviral vectors, CRISPR-Cas9 genome engineering.
- A comprehensive PhD training program including lectures, workshops, meetings and retreats organized by CRC854.
Your skills and qualities:
- A master degree (or equivalent qualification), preferably in (Bio) Medicine, Molecular Biology, Cell Biology, Immunology or a related discipline
- Experience in one or more of these techniques is desirable: Immunology/immunobiological techniques, Molecular Biology/Genetics, Cell culture.
- Good organizational and communication skills, self-motivation, team player skills, good language skills in English.
Please include the following in your application: a Motivation letter, CV, copy of graduation certificates, copy of University grades, any other relevant documents, and contact details of one or two references.
Please submit your application, preferably as a PDF by E-Mail to firstname.lastname@example.org and Cc to email@example.com.
Prof. Dr. med. Thomas Tüting
Department of Experimental Dermatology/ Clinic of Dermatology and allergy
Otto von Guericke University
Leipziger Strasse 44
D- 39120 Magdeburg.